Molecular cloning of human interleukin 2 cDNA and its expression inE. coli
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چکیده
منابع مشابه
Molecular cloning of human interleukin 2 cDNA and its expression in E. coli
hIL-5 cDNA was amplified through reverse transcription-polymerase chain reaction from peripheral blood lymphocytes induced with PMA and calcium ionophore A23187. The hIL-5 fragment was cloned into pUC18 and its sequence was identified to be hIL-5 cDNA sequence. The fragment which encodes hIL-5 mature polypeptide was amplified and cloned into pBV220 to express hIL-5 recombinant protein in E. col...
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Interleukin-2 (IL-2 or T cell growth factor) is a 14,500 daltons glycoprotein critically involved in the development of a normal human immune response [1, 2J. Recently, cDNA for this lymphokine has been isolated and expressed in both prokaryotic and eukaryotic cells [3-5J. Further, the human IL-2 gene has been cloned, sequenced [6], and localized to chromosome 4. As with other polypeptide hormo...
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Prior to the production of human gamma interferon using recombinant DNA technology, it had been producedmainly upon mitogenic induction of lymphocytes in very low amounts, which evidently hamperedits characterization and its medical applications. The recombinant gamma interferons produced in largerquantities in prokaryotic systems retain their biological activities, and can be...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1983
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/11.13.4307